Related or semi-related downstream processing delivers sizeable advancements to efficiency, Price tag, timelines, and facility footprint. Our flexible portfolio allows you select an intensification method that aligns with your special method objectives.
Pumps vary in stress potential, but their effectiveness is measured on their capability to generate a reliable and reproducible volumetric move fee.
An economical, biospecific bond is fashioned by a simultaneous and concerted motion of quite a few of those forces in the complementary binding internet sites.
Gradient elution is a technique used to optimize the separation of advanced mixtures in HPLC. It involves altering the composition of your cell stage throughout the analysis.
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The retention factor is calculated by multiplying the distribution frequent by the amount of stationary phase while in the column and dividing by the quantity of mobile phase within the column.
Sizing-Exclusion HPLC: Dimension absence Chromatography (SEC) is really a chromatographic procedure that only distinguishes between molecules based mostly on their dimensions. In this process, molecules are divided with the column packing substance based mostly on their own absence from holes.
Enables simultaneous and continuous operation of up to a few chromatography separations. These is often Component of a batch and/or multi-column course of action
The choice from the mobile period is crucial in HPLC mainly because it decides the elution buy check here and separation performance. Normally made use of cellular phases incorporate mixtures of water here and organic solvents including acetonitrile or methanol.
The place B is the compound that is retained extra strongly by the column plus a is the compound Using the faster elution time.
Enables larger productivity than conventional chromatography, reduced buffer and resin volumes and also reduced resin prices
Sign up for Sartorius as we take a look at ways to transfer a standalone batch mAb chromatography course of action to a linked DSP.
Other attributes which can be analysed include the amounts of glycans, amino acids and peptides. This is certainly discussed in more element within the report ‘Strategies for your Characterisation of Biopharmaceuticals’.
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